Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azideData: Availability 1-3 business days Species Reactivity Human, Mouse, Primate, Rat Format Purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Purified UniProt P14618 Applications Immunofluorescence : 1:200Western blot : 1:1000IHC (Paraffin) : 1:50-1:1:100 Limitations This PKM2 antibody is available for research use only. Description:PKM2 is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]Application Notes:Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.Immunogen:A portion of amino acids 476-505 from the human protein was used as the immunogen for this PKM2 antibody.Storage:Aliquot the PKM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.