Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name rWT1/857 Purity Protein G affinity chromatography UniProt P19544 Localization Cytoplasmic, membranous Applications Flow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 0.5-1ug/mlImmunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT Limitations This recombinant WT1 antibody is available for research use only. Description:Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.Immunogen:A recombinant human protein was used as the immunogen for the recombinant WT1 antibody.Storage:Store the recombinant WT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).