DescriptionSynaptosome-associated protein of 25,000 daltons also known as SNAP-25 is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE(the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa(SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E(BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms(a and b) have been described, which are produced by alternative splicing. Nagy et al.(2004) identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.Application NotesThe stated application concentrations are suggested starting amounts.Titration of the SNAP25 antibody may be required due to differences in protocols and secondary/substrate sensitivity.ImmunogenAn amino acid sequence from the C-terminus of human SNAP25 (DSNKTRIDEANQRAT) was used as the immunogen for this SNAP25 antibody (100% homologous in human, mouse and rat).StorageAfter reconstitution, the SNAP25 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing. Related Products1. View all SNAP25 antibodies