Pre-made lentiviral particles bicistronically express two individual proteins: firefly luciferase and nuclear permeable CRErecombinase, under the same suCMV promoter. A Puromycin marker was expressed under RSV promoter, which allow to select the positive transduced stable cells for long term culture via antibiotic selection.

Both lucifease and CRE are highly active, best for both enzymes\' assays In vivo or In vitro. CRE protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,named lox sites. CRE lentiviral particlescancatalyzethe jointing event In Vivoin the presence oflox sites. Thisnuclear permeantCRE (NLS-CRE)increases the recombination events in vivo.Purified CRE enzyme can joint individual plasmids containing lox sites.

Luciferae and NLS-CREare constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expressionas desired. Luciferase signal provides a convenient, realtime monitoring theparticles performance.

See product manual for details (.pdf).

Amount:200ul/per vial, at1 x 10⁷ IFU/ml in DMEM medium with 10% FBS.

Cat#: LVP409