Pre-made lentiviral particlesexpressing sequence verified CRErecombinase under induciblesuCMV promoter. CRE contains a NLS (nuclear localization signal)at its N-term. Thisnuclear permeantCREincreases the recombination events in vivo.A Puromycin marker underRSV promoter, allows to select transduced cells for long term expression via blasticidin antibiotic.
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,named lox sites. CRE lentiviral particlescancatalyzethe jointing event In Vivoin the presence oflox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
NLS-CRE is constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expressionas desired.See product manual for details (.pdf). Amount:200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS. Cat#: LVP339
