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Immunoprecipitation of Myc-tagged proteins: Myc-Trap, an anti-Myc-tag Nanobody coupled to agarose, (left) is compared with a conventional anti-Myc-tag antibody coupled to agarose (right). Whereas Myc-Trap binds only the Myc-tagged protein (lane B, bound) there are also heavy and light antibody chains present in the bound fraction (lane B) of the conventional antibody resin.

Description The ChromoTek Myc-Trap® is an affinity resin for immunoprecipitation and purification of Myc-tagged proteins.

It comprises an anti-Myc-tag Nanobody/ VHH coupled to beads.

Specificity The Myc-Trap recognizes the Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. The Myc-Trap does not bind endogenous c-myc.

Applications Immunoprecipitation (IP) / Co-IP

Affinity purification Mass spectrometry (MS) On-bead enzyme assays ChIP/ RIP analysis

Formats/matrices Myc-Trap Agarose Myc-Trap Magnetic Agarose Myc VHH, recombinant binding protein iST Myc-Trap Kit for IP plus sample preparation for MS

Synonyms Myc Nanobody, anti-Myc VHH, Myc-binding protein or Myc single domain antibody fragment (sdAb)

Efficient immunoprecipitation of Myc-tag fusion proteins, low background No heavy light antibody chains in downstream applications Easy elution of native proteins Harsh washing conditions

We offer the anti-Myc VHH coupled to agarose beads and magnetic agarose beads, or uncoupled as purified protein.

Myc-Trap Agarose Myc-Trap Magnetic Agarose matrix agarose magnetic agarose color white black particle size 90 µm 40 µm magnetic no yes may be centrifuged up to 2,500 x g 800 x g Dissociation constant KD of 500 nM for 1x Myc Dissociation constant KD of 0.5 nM for 2x Myc Compatible to harsh wash conditions such as 2 M NaCl, 2 % Nonidet P40 Substitute Applications validated and characterized

The Myc-tag is derived from the human c-myc oncogene. It is a commonlyused epitope tag to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Myc-tag is also named c-myc tag.

The sequence motif is EQKLISEEDL.