首页 > FormuMax > FormuMax/Trans-Hi™ - In vitro Transfection Reagent/1.0 mL/F90101TH-10

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FormuMax/Trans-Hi™ - In vitro Transfection Reagent/1.0 mL/F90101TH-10

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F90101TH-10

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产品说明

Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.Key Features for Trans-HiTMExcellent transfection for single DNA transfection or multi DNA co-transfectionRapid onset and long-lasting transfectionSuitable for very long DNAsSimple and easy to use transfection procedureRobust and reproducible transfectionHigh level of recombinant protein productionVery cost efficientLong shelf-life: 12 monthsSpecifications: Product type:In vitro cell transfection reagentApplicationsPlasmid transfection, protein overexpressionTechnique:Polymer-based DNA nanoparticlesCell type:Established cell lines, primary cells, hard-to-transfect cellsAdherent cells and suspension cellsSample type:Plasmid DNASerum compatibility:YesProduct size:0.5, 1.0 and 10x1.0 mLNumber of transfectionsUp to 660 in 24-well plates with 1.0mL reagentup to 330 transfections in 6-well platesShelf-life:12 months or longer when store refrigerated (2-8°C)Shipping condition:Room temperatureProtocolsTrans-Hi™General Protocol For In Vitro Transfection in Mammalian CellsTrans-Hi™ - Protocol for Transfecting 293 and CHO Suspension CellsTrans-Hi™ - Protocol for Generation of rAAVTrans-Hi™-Technical Tips For Good In Vitro TransfectionComparison study ofIn vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine®2000in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per wellused for both products in 24 well plate 30 hours post transfection.Trans-Hi™/DNA ratio (µL/µg) (HEK293T)Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in24 well plate 30 hours post transfection, 0.5ug DNA per well).Head-to-head comparisonTrans-Hi™ against Lipofectamine 2000HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection). Trans-Hi™Lipofectamine-2000TimeDay 1Day 2Day 3Day 1Day 2Day 3HEK293A (%)79.593.493.375.789.450.6Comparison study ofIn vitroplasmid DNA transfection of Trans-Hi™and Lipofectamine®2000in Hela cellswith GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well),24 well plate 48 hours post transfection).Broad Transfection Spectrum for Mammalian Cell TypesCell LinesEfficiency (% GFP)COS-785 - 90%Hela85 - 90%HEK-293T85-90%NIH 3T375%A54975%Hep3D65-75%SHEP65-70%CaCo260%HepG270%SKOV365%MCF-765-70%3T3-442A35-40%CHO80-90%

FormuMax科学公司位于加利福尼亚州北部硅谷的中心地带，是一家专业的药品配送公司，致力于满足医药/生物技术、化妆品和保健工业的配方开发外包的日益增长的需求。经营理念是成为**的伙伴，作为药物交付的***，特别是在制定具有挑战性的化合物脂质体和其他新的纳米技术。FormuMax拥有一支技术团队，在药物递送领域拥有***的专业知识，特别关注脂质体和其他基于脂质的技术。它们目前提供基础研究，配方可行性，定制配方准备，配方表征和分析科学的合同服务。FormuMax是**家提供大量预成型脂质体试剂的在线商店。已经成功地为来自世界各地的大学实验室、研究中心和各种行业的客户提供了服务和/或产品。

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