GenWay/Anti-MPO (p-ANCA)/GWB-521206/1x96 Assays
Description:
Introduction| Anti-neutrophilic-cytoplasm antibodies (ANCA) represent a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods.With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type. The main antigen for the c-ANCA is the proteinase 3 (PR3), which is a serine proteinase of the present in primary granules.Antibodies of p-ANCA positive sera are mainly directed to myeloperoxidase (MPO). Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin-G and also lysozyme often result in a similar p-ANCA pattern. Beside different untypical variants of p-ANCA IF patterns granulocyte specific antinuclear antibodies (GS-ANA) is indistinguishable from p-ANCA. This makes a clear interpretation and classification of the IF patterns difficult. Therefore every positive IF-ANCA findings esp. p-ANCA should be differenciated by ELISA techniques using purified antigens. A survey of documented clinical indications of specific ANCA is given in the table below. PR3-ANCA and MPO-ANCA are reliable serologic markers in the diagnostics of vasculitides. PR3-ANCA is the classical autoantigen in Wegener´s granulomatosis with a clinical specificity of more than 95%. c-ANCA is documented to be present in different diseases. Anti-MPO antibodies are highly specific for idiopathic and vasculitis associated crescentic glomerulonephritis and also for classic polyarteritis nodosa, Churg-Strauss syndrome and the polyangitis overlap syndrome without renal involvement.7-10 With respect to sensitivity, either MPO or PR-3 antibodies were found in 77 to 100% of patients with idiopathic and vasculitis associated crescentic glomerulonephritis. In WG, anti-MPO antibodies were detected only occasionally and generally in patients negative for PR-3 antibodies. The MPO and PR-3 specific ELISA methods can provide an important confirmatory result for two of the more important of the identified antigens. ELISA is also useful for interpreting \"difficult\" samples by IFA such as those which exhibit several antibodies simultaneously or those with high background fluorescence. |
Intended Use| Anti-MPO (p-ANCA) is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies against myeloperoxidase (MPO) in human serum or plasma. The assay is intended research use only as an aid in the diagnosis of certain autoimmune vasculitites such as Wegener´s granulomatosis. Anti MPO (p-ANCA) kit is intended for laboratory use only. |
Principle of the Assay| The anti-MPO IgG test is based on the binding of present antibodies on the human neutrophil myeloperoxidase coated on themicroplates. Any present antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. Aftera 60 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components.An anti-human-IgG horseradish peroxidase conjugate solution recognize IgG class antibodies bound to the immobilized antigens.After a 30 minutes incubation any excess enzyme conjugate, which is not specifically bound is washed away with wash buffer.A chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color developmentis stopped by adding the stop solution. The solutions color change into yellow. The amount of color is directly proportional to theconcentration of IgG antibodies present in the original sample. |
Additional Information:
Name | Anti-MPO (p-ANCA) |
Related Product Names | Anti Myeloperoxidase (MPO) ELISA, Anti Myeloperoxidase ELISA, Anti MPO ELISA, Perinuclear Anti-Neutrophil Cytoplasmic Antibodies (p-ANCA) ELISA Kit, Perinuclear Anti-Neutrophil Cytoplasmic Antibodies ELISA, p-ANCA ELISA, ANCA / Vasculitis Assay |
Molecular Weight | 0.5 |
Concentration | Lot Specific |
Applications | Enzyme immunoassay for the quantitative determination of anti MPO in human serum or plasma |
Reactivity | Human |
Storage | The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C in the dark. |
Intended Use | Research Use Only |
