GenWay/Mouse SAA ELISA Kit/GWB-DB6944/96 well
Additional Information:
Name | Mouse SAA ELISA Kit |
Related Product Names | Mouse SAA ELISA Kit |
Gene Symbol | SAA1 |
Gene Fullname | serum amyloid A 1 |
Gene id | 20208 |
Alias Symbols | Serum amyloid A-1 protein, SAA |
Protein Name | Serum amyloid A-1 protein |
Description of Target | Major acute phase protein. |
Swissprot ID | P05366 |
Species Reactivity | Mouse |
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Sensitivity | 11.015 ng/ml |
Kit Range | 31.25 ng/ml - 2,000 ng/ml |
Homology | Mouse |
Standard Range | Refer To Manual |
Sample Type | Serum, plasma and other biological fluids |
Product Format | In Solution |
Specimen/Volumes | Refer To Manual |
Applications | ELISA |
Reconstitution and Storage | 1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-SAA conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-MOUSE SAA ELISA MICRO PLATEAnti-Mouse SAA coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. MOUSE SAA CALIBRATORThe lyophilized Mouse SAA calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
Reactivity | Mouse |
Kit Size | 96 well |
Kit Principle | Genway Biotech SAA ELISA Kit (Mouse) (GWB-DB6944) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum amyloid A-1 protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum amyloid A-1 protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum amyloid A-1 protein captured in the well. |
Kit Component ComponentAmount | Anti-Mouse SAA Microplate | 96 Wells (12 x 8 Well Strips) | | 20X Diluent Concentrate | 50 mL | | 20X Wash Solution | 50 mL | | 100X Enzyme-Antibody Conjugate | 150 uL | | Chromogen-Substrate Solution | 12 mL | | Stop Solution | 12 mL | | Mouse SAA Calibrator | 1 |
|
Kit Duration | 100 Minutes |
Storage | Refer to kit instructions for component storage |
Lead Time | Domestic: within 1 week deliveryInternational: 1 week |
Intended Use | Research Use Only |
Additional Information | Intended Use: The SAA test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in biological samples of mice. |
:: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Mouse SAA antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-MOUSE SAA ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse SAA.7. MOUSE SAA CALIBRATOROne vial containing a lyophilized Mouse SAA calibrator. |
