Description:

Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Additional Information:

Name Related Product Names Short Description Immunogen Standard Range Sample Type Specimen/Volumes Swiss Prot Number Concentration Applications GI Number Stability Reactivity Kit Size Storage Intended Use

Rat GM-CSF ELISA Kit

Colony stimulating factor|Colony stimulating factor 2 (granulocyte macrophage)|CSF|CSF 2|CSF2|GMCSF|Granulocyte macrophage colony stimulating factor|MGC131935|MGC138897|Molgramostin|Sargramostim

Sandwich ELISA kit of Quantitative Detection for Rat GM-CSF

E.coli, A1-K127

15.6pg/ml-1000pg/ml. Test has a sensitivity of

This kit is used for quantitative detection of rat GM-CSF in cell culture supernates, serum and plasma(heparin, EDTA).

Refer to Manual

P48750

Lot Specific

ELISA

116630

Four months at 4C and eight months at -20C.

Reactive to Rat. There is no detectable cross-reactivity with other relevant proteins.

1x96 well plate

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Research Use Only