Description:

Description: Human GSK3beta GenBank Accession No. NM_002093), full length, with N-terminal GST tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 391 pmol/min/ug. Enzyme reaction was conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 9, Invitrogen) at room temperature for 1 hour. Developer solution was added and reaction was stopped after 1h of incubation at RT. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability :>6 months at -80°C. References: 1. Sutherland C. et al. Biochem J. 1993 Nov 15;296 ( Pt 1):15-9. 2. Sperber BR et al. Neurosci Lett. 1995 Sep 8;197(2):149-53. Host: baculovirusTag: N-terminal GST tagGI Num: NM_002093MW: 73 kDaFormat: aqueous buffer solutionFormula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathioneScientific Category: Kinase/Serine-ThreonineSpecies: human

Additional Information:

Name Related Product Names Molecular Weight Purity Source GI Number Format Reactivity Storage Intended Use

GSK3beta, Active Human Recombinant Protein

GSK3beta, Active Human Recombinant Protein

73 kDa

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

baculovirus

NM_002093

Aqueous Buffer Solution

Human

-80°C

Research Use Only