Description:
Description: Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity. Summary: - Detection method- Flow cytometry (400 nm excitation filter and 505 nm emission filter)and fluorescence microscopy - Sample type- Cell and tissue lysates- Species reactivity- Mammalian - Kit size- Convenient sizes (25, 100, 200, 400 assays)- Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.Kit components: - Cell Lysis Buffer- 2X Reaction Buffer- YVAD-AFC (1mM)- DTT (1 M)Features and Benefits: - Simple one-step procedure; takes 1-2 hours- Fast and convenient- Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity. Shipping Conditions: Gel pack
Additional Information:
Name | Caspase-1 Fluorometric Assay Kit - 200 assays |
Related Product Names | caspase1, caspase 1, Caspase-1 Fluorometric Assay Kit, Caspase-1, Caspase-1 Substrate, YVAD, YVAD-AFC, Caspase-1 assay, Caspase-1 Fluorometric Assay |
Format | Assay Kit |
Storage | -20C |
Intended Use | Research Use Only |
