Description:

Description: Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal HIS6 tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells. Stability :>6 months at -80°C Purity: >70 % by SDS-Page Specific Activity: 100 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 0.3 mg/ml MBP substrate, and 0.2 ug/ml NEK2. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. References: 1. Fletcher, L. etal., Biochim. Biophys. Acta 1744 (2), 89-92 (2005) 2. Fardilha, M. et al., Ann. N.Y. Acad. Sci. 1030, 468-478 (2004).Host: baculovirusTag: N-terminal His tagGI Num: NM_002497MW: 53 kDaFormat: aqueous buffer solutionFormula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazoleScientific Category: Kinase/Serine-ThreonineSpecies: human

Additional Information:

Name Related Product Names Molecular Weight Purity Source GI Number Format Reactivity Storage Intended Use

NEK2, Active Human Recombinant Protein

NEK2, Active Human Recombinant Protein

53 kDa

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

baculovirus

NM_002497

Aqueous Buffer Solution

Human

-80°C

Research Use Only