Description:

Please click here for MSDS PDF DatasheetProduct Name: Trichinella spiralis IgG – ELISA# of Samples: 1 x 96 AssaysIntended Use: The Trichinella spiralis IgG-ELISA is intended for the qualitative determination of IgG class antibodies against Trichinella spiralis in human serum or plasma (citrate). Introduction: Trichinosis (also called trichinellosis) is caused by nematodes (roundworms) of the genus Trichinella. In addition to the classical agent Trichinella spiralis, which is found worldwide in many carnivorous and omnivorous animals, four other species (T. pseudospiralis, T. nativa, T. nelsoni, and T. britovi) are recognized. Trichinosis is acquired by ingesting meat containing cysts of Trichinella. After exposure to gastric acid and pepsin, the larvae are released from the cysts and invade the small bowel mucosa where they develop into adult worms (female 2.2 mm in length, males 1.2 mm). After 1 week, the females release larvae that migrate to the striated muscles where they encyst. Encystment is completed in 4 to 5 weeks and the encysted larvae may remain viable for several years. Ingestion of the encysted larvae perpetuates the cycle.Trichinosis infection occurs worldwide, but is most common in parts of Europe and the United States. Light infections may be asymptomatic. For mild to moderate infections, most symptoms subside within a few months whereas fatigue, weakness, and diarrhoea may last for months. In severe cases, death can occur.Infection may be identified byMicroscopy, muscle biopsySerology: Detection of antibodies by ELISAPrinciples of the assay: The qualitative immunoenzymatic determination of antibodies against Trichinella spiralis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Trichinella spiralis ES-antigens (Excretory/Secretory antigens) to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A Conjugate is added. This conjugate binds to the captured Trichinella spiralis-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Trichinella spiralis specific antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance alternative 450 nm is read using an ELISA microwell plate reader.Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history, symptomatology as well as serological data.In immunocompromised patients and newborns serological data only have restricted value.Cross reactions with antibodies against Toxocara canis are possible. References Despommier, D.: Trichinellosis. Immunodiagnosis of Parasitic Diseases, Vol. 1, Helminthic Diseases. Ed. Walls and Schantz. Academic Press, 1986, pp. 163-181.Krogstad, D., Visvesvara, G., Walls, R., Smith, J.: Tissue Helminths. Manual of Clinical Microbiology, 4th Ed. American Society for Microbiology, Washington, DC. 1985, pp 654.Kagan, I.: Serodiagnosis of Parasitic Diseases. Manual of Clinical Laboratory Immunology. 3rd Ed.. American Society for Microbiology, Washington,DC., 1986, pp. 474-477.Petri, W. et al.: Common-Source Outbreak of Trichinosis Associated with Eating Raw Home-Butchered Pork. So Medical J, August 1988, pp. 1056-1058.Oliver, D. et al.: Enzyme Linked Immunoassay for Detection of Trichinosis in Humans. Proc 7th Int Conf on Trich, Oct. 1988.Ivanoska, D. et al.: Comparative Efficacy of Antigen and Antibody Detection Tests for Human Trichinellosis. J Parasit, Vol 75#1, 1989, pp. 38-41.

Additional Information:

Name Related Product Names Molecular Weight Storage Intended Use

Trichinella spiralis IgG (Trichinosis)

Trichinella spiralis IgG – ELISA

0.5

The reagents are stable up to the expiry date stated on the label when stored at 2...8 °C.

Research Use Only