应用
病原体减少治疗间充质干细胞免疫细胞可规模化制造病原体减少治疗
UltraGRO™ 产品含有细胞生长所需的丰富生长因子和细胞因子，被公认为是用于制造细胞和细胞产品的 FBS 的高效无异种替代品。
符合 cGMP考虑到病原体风险评估，所有 UltraGRO™ 产品均来自 FDA 许可的中心。每个捐赠者都经过采访、评估，并且使用 FDA 许可的传染病筛查测试对捐赠进行了测试。在确定捐赠适合使用之前，所有捐赠者的材料必须对所有经过筛查的病原体进行阴性测试。为了进一步最大限度地降低病原体传播的风险，FDA 许可的中心建立了详细的跟踪系统AventaCell 收到的所有材料都将经过验证和记录，然后保留一段保留期。如果捐赠在收集时检测为阴性，但捐赠者在下一次捐赠中检测为阳性，则库存保留期允许 FDA 许可的中心通知系统有机会帮助排除任何可疑单位用于制造。此外，我们的cGMP生产系统可防止生产过程中发生外来病原体污染。
此外，由于这些\"窗口期”供体在采集供体时无法进行筛选， AventaCell 采用最先进的伽马射线辐照工艺作为病毒灭活的病原体减少处理 (PRT)，以创建 UltraGRO™ GMP PRT 产品。成品的伽马射线照射会破坏病毒基因组的核酸并使其感染能力失活。我们已经验证了伽马射线照射工艺的有效性病毒灭活，同时对我们的 UltraGRO™GMP PRT 产品的效力和长期稳定性影响很小。
UltraGRO™ GMP PRT 产品提供更高的安全标准，最大限度地降低病原体污染风险，同时保留人类 MSC 的有效细胞培养性能、免疫细胞和其他适用于临床和商业应用的细胞类型。
主页>产品>UltraGRO™所有产品UltraGRO™-PURE GIUltraGRO™-Advanced GIUltraGRO™-PUREUltraGRO™-AdvancedUltraGRO™产品
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UltraGRO™ GMP 级不含异种物质
细胞培养补充剂
GMP 级
不含异种成分
细胞培养补充剂
研究级< /p>1
主页>产品>UltraGRO™-Advanced所有产品UltraGRO™-PURE GIUltraGRO™-Advanced GIUltraGRO™-PUREUltraGRO™-AdvancedUltraGRO™产品
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UltraGRO™-高级 GMP 级非异种（原始材料）
去纤维蛋白原细胞培养补充剂
GMP 级
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UltraGRO™-高级研究级非异种（原始材料）
< p>纤维蛋白原耗尽的细胞培养补充剂研究级
1主页>产品>UltraGRO™-PURE所有产品UltraGRO™-PURE GIUltraGRO™-Advanced GIUltraGRO™-PUREUltraGRO™-AdvancedUltraGRO™产品
![\"\"](\"https://www.atcbiomed.com/./uploads/ct/products/20201019892.jpg)
UltraGRO™-PURE GMP 级不含异种物质
去纤维蛋白原细胞培养补充剂
GMP 级
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UltraGRO™-PURE 研究级不含异种
纤维蛋白原耗尽的细胞培养补充剂< /p>
研究级
1主页>产品>UltraGRO™-Advanced GIAll 产品UltraGRO™-PURE GIUltraGRO™-Advanced GIUltraGRO™-PUREUltraGRO™-AdvancedUltraGRO™产品
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UltraGRO™-高级 GI GMP 级非异种（原始材料）
伽马射线照射的纤维蛋白原耗尽细胞培养物
GMP 级
1主页>产品>UltraGRO™-PURE GIAll 产品UltraGRO™-PURE GIUltraGRO™-Advanced GIUltraGRO™-PUREUltraGRO™-AdvancedUltraGRO™产品
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UltraGRO™-PURE GI GMP 级不含异种
伽马射线照射的纤维蛋白原耗尽细胞培养物
GMP 级
1Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented. Western blot analysis was performed on whole cell extracts (50 µg lysate of Jurkat (lane 1), Jurkat treated with TNF-alpha (50 ng/mL/20 min) (lane 2) and Jurkat treated with Calyculin A (50 ng/mL/20 min) (lane3). The blots were probed with Anti-NF-kappa-B p65 (pS536)Rabbit Polyclonal Antibody (Product # 720006, 2-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 65 kDa band corresponding to NF-kappa-B p65 (pS536)was observed across cell lines tested according to the treatment. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Immunofluorescence was performed on fixed and permeabilized Jurkat cells treated with Calyculin A (50 ng/mL/20 min) for detection of NF-kappa-B p65 (pS 536) using Anti-NF-kappa-B p65 (pS536)Rabbit Polyclonal Antibody (Product # 720006, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of NF-kappa-B p65 (pS536) protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of NF-kappa-B p65 (pS 536). Panel e) represents control cells with no primary antibody to assess background. 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit and Dog. Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. Phosphorylation of p65 at Ser 536 located in the carboxy-terminal transactivation domains (TAD) leads to nuclear localization of the transcriptionally active complex and NF-kB mediated transactivation of several downstream genes. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells. Have you cited this product in a publication? Let us know so we can reference it here. Cite this product 蛋白别名: MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A Host server : magellan-srch-1-prod-green:8080/10.253.224.248:8080. git-commit: 2cd8645d2fc6bfe4ccb4abfa14772b0a94f68e98 git-url: http://victoria.invitrogen.com:8333/magellan/core.git git-branch: origin/release/1.27.0-2021.08.32-1.0