Description: Amyloid-beta (N-term), Human, mAb VU17
Antibody clone VU17, formerly known as αVU Aß 17, recognizes the N-terminus of human amyloid beta (i.e. in: Aβ1-38; Aβ1-39; Aβ1-40; Aβ1-42). Amyloid beta peptides are of key importance in the development of Alzheimer’s disease (AD) and are present in CSF. AD affects approximately 1% of the population over the age of 80. The disease is characterized by aggregation of Aβ into oligomers, fibrils and plaques deposition of insoluble and aggregated protein in the brain where its neurotoxic action leads to neuronal cell death. The deposits, also called amyloid plaques, are formed around the neuropil. The exact molecular mechanism is still unclear. Aβ is a proteolytic cleavage product, by APP-cleaving enzyme 1 (BACE1) and β-secretase complex, of membrane bound amyloid precursor protein (APP). Amyloid is the term used for proteins that are misfolded into a cross β-sheet structure. Aβ is produced by various cell types and is secreted in the interstitial fluid. There are multiple cleavage sites in Aβ domain leading to various fragments of 36-43 amino acids in length. The main peptide in the CSF terminate at residue 40. CSF contains many Aβ isoforms, of which Aβ40 is approximately 10 times more abundant than Aβ42. Aβ40 levels are unchanged in AD, but there is a change in Ab40:Aβ42 ratio in AD. This ratio has been thought of diagnostic importance. Assessment of Aβ in brain tissue is essential for diagnosis and is crucial for investigating the molecular mechanisms underlying AD. Antibody VU17 is raised against the N-terminus of Aβ. The antibody detects all forms of Aβ deposits without the need for formic acid pre-treatment on paraffin coupes and can be applied in a double staining strategy, making it suitable for investigating co-localization.
Specifications:
Catalog number | HM2325 |
Product type | Monoclonal antibodies |
Quantity | 100 µg |
Formulation | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
Application | Frozen sections, Immuno assays, Immuno precipitation, Paraffin sections |
Application Notes | IHC-P: Fixation is performed using 4% paraformaldehyde. VU17 can be used for double staining (Ref1). W: The antibody is very specific and sensitive for the last C-terminal amino acid of synthetic Aβ1-40. No cross-reactivity was seen with other Aβ forms (Ref2). |
Use | The typical starting working dilution is 1:50. Dilutions have to be optimized in user’s experimental setting. |
Isotype | Mouse IgG2a |
Species | Human |
Alias | Abeta, Aβ, β-Amyloid |
References | 1. Verwey, N et al; Immunohistochemical characterization of novel monoclonal antibodiesagainst the N-terminus of amyloid β-peptide. Amyloid 2013, 20:3 2. Verwey, N et al; Quantification of amyloid beta 40 in cerebrospinal fluid. Journal of Immunology methods 2009, 348:1-2 |
Storage and stability | Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. |
Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
Disease | Cardiology and metabolism, Neurological disorders |
