Description: TNF-alpha, Human, mAb 5N
The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha.
Specifications:
Catalog number | HM2218 |
Product type | Monoclonal antibodies |
Quantity | 100 µg |
Formulation | 1 ml (500 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin. |
Application | Functional studies, Immuno assays |
Use | For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10-10M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha. |
Isotype | Mouse IgG2a |
Species | Human |
Cross reactivity | Bovine - Yes, Canine - Yes, Monkey - Yes |
Alias | TNF, TNF-SF2, DIF, Cachectin |
References | 1. Petyovka, N et al; Homologous ELISA for detection of oligomeric human TNF: properties of the assay. J Immunol Methods 1995, 186: 161 2. Song, X et al; Effects of 2 different anti-tumor necrosis factor-alpha agents in a primate model of subcutaneous formation. J Infect Dis 2002, 185: 204 |
Storage and stability | Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. |
Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
Disease | Autoimmunity, Gastroenterology |
