Description: MASP-2, Human, mAb 8B5

The monoclonal antibody 8B5 reacts with the CCP1/2-SP fragment of human MASP-2. MASP-2 is a trypsin-like serine protease and plays an important rol in the initiation of the MBL complement activation pathway. Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component C3. In the lectin pathway, mannose binding-lectin (MBL)-associated serine proteases (MASPs) form complexes with polymeric lectin molecules which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of micro organisms, MASPs are converted to their active forms and initiate complement activation. Three types of human MASP have been reported. MASP-1, MASP-2 and MASP-3. Mannan-binding lectin (MBL) and ficolins, in complex with MBL-associated serin proteases (MASPs), are capable of activation the complement system, thus mediating the destruction of infectious agents. MASP-2 cleaves C4 and C2 and is crucial for the activation of downstream complement components.

Specifications:

Catalog number Product type Quantity Formulation Application Use Isotype Species References Storage and stability Precautions Disease

HM2190

Monoclonal antibodies

100 µg

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Immuno assays, Western blot

For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Rat IgG1

Human

Moller-Kristensen, M et al; Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals. J Immunol 2003, 282: 159

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Infectious diseases