Produce fully RNase A-resistant RNA that is perfectly suited for RNA aptamer synthesis, as well as antisense RNA and RNA interference (RNAi) experiments.

• Protect transcribed RNA from RNAse degradation
• Produce high RNA yields
• Select and optimize RNA aptamers using SELEX screening
• See FAQ

The DuraScribe® T7 Transcription Kit* is an in vitro transcription kit that produces RNA - called DuraScript® RNA - that is completely resistant to RNase A digestion.

The DuraScribe T7 Transcription Kit includes DuraScribe T7 RNA Polymerase, an enhanced formulation of the Y639F mutant¹ of T7 RNA Polymerase that efficiently incorporates 2´-Fluorine-CTP (2´-F-dCTP), 2´-Fluorine-UTP (2´-F-dUTP), ATP and GTP into RNase-resistant RNA transcripts called DuraScript RNA (Figure 1). The DuraScribe T7 RNA Polymerase uses the same T7 promoters as the wild-type T7 RNA Polymerase.

Applications

DuraScript RNA has demonstrated advantages for RNA aptamer synthesis, and ribozyme, RNA interference (RNAi) and antisense RNA studies.

Features

• DuraScript RNA is resistant to Rnase A and Dnase
• High Yields of DuraScript RNA
• T7 in vitro transcription reations. DuraScribe T7 RNA Polymerase uses the same T7 promoters as the wild type T7 RNA Polymerase.
• Ideal for RNA aptamer studies. Numerous citations using the DuraScribe Kit with RNA aptamers
• SELEX-compatible. DuraScript RNA can be used in SELEX selection processes.
• Make long or short DuraScript RNA. Double-strand oligos, linearized plasmids, and PCR products with a T7 polymerase promoter can be transcribed.

Table 1. Yield of DuraScript® RNA from a DuraScribe® Kit reaction. One microgram of a 3-Kb DNA template was linearized at different sites and then transcribed in a DuraScribe T7 Transcription Kit reaction for 4 hours. The yield of DuraScript RNA produced from each template is shown in micrograms (µg) and in picomoles (pmol).

* Covered by issued and/or pending patents.

References

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