Optimized for high signal intensity
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Fast. Perform the 6-hour reaction and begin BeadChip hybridization the same day. - High Sensitivity. Sensitive and reproducible gene detection.
- High Yield. Make microgram amounts of biotin-aRNA (biotin-cRNA) from as little as 25 ng of total RNA.
- High Fidelity. TargetAmp-Nano labeling procedure preserves the relative abundance of the original sample.
The TargetAmp™-Nano Labeling Kit produces biotin-aRNA (also called biotin-cRNA) that has been optimized for high signal intensity on Illumina Expression BeadChip arrays, enabling sensitive gene detection and reproducible differential expression results.
Benefits
- Fast. Perform the 6-hour reaction and begin BeadChip hybridization the same day.
- High Sensitivity. Sensitive and reproducible gene detection.
- High Yield. Make microgram amounts of biotin-aRNA (biotin-cRNA) from as little as 25 ng of total RNA.
- High Fidelity. TargetAmp-Nano labeling procedure preserves the relative abundance of the original sample.
Table 1. TargetAmp-Nano Kit aGuide
| Input RNA | Reaction Time | Additional | |
| TargetAmp-Nano Kit | 25 ng - 500 ng total RNA | 6 hours | Requires use of SuperScript III Reverse Transcriptase (provided by the user) |
Table 2. Yield of Biotin-aRNA from TargetAmp-Nano Kit
| Input RNA (HeLa total RNA) | 50 pg | 100 pg | 500 pg | 25 ng | 100 ng | 500 ng |
| TargetAmp-Nano Kit reaction | 3 μg | 10 μg | 75 μg |
