• Excellent strand displacement, fidelity and speed.

phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

An example of the savings you can expect.

Specifications

Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTPs into acid insoluble material in 10 minutes at 30°C.

Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.

ORDER INFORMATION

Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH₄)₂SO₄, 40 mM Dithiothreitol, 100 mM MgCl₂, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.