北京市昌平区北清路生命科学园北清创意园2-1-102; Tel：010-88891086，80794780 Fax：010-80794780咨询订购QQ: 1443274331 技术支持QQ: 2074750152 邮件订购：bjzblg@126.comwww.epibio.com.cn www.epicentre.cn www.immunoreagents.cn www.lucigen.com.cn版权所有 @北京中北林格科技发展有限公司 京ICP备：13002510号 网站建设：中企动力 北京 感受态细胞选择指南   Blue-whitecapablPhageT1resistantEndA-/RecARestriction-deficient(RMS)ClonemethylatedDNAInducibleplasmidcopynumberF’episomeAntibioticresistanceElectrocompetent:efficiencyChemicallycompetent: Blue-white capablPhage T1 resistantEnd A -/RecARestriction-deficient (RMS)Clone methylated DNAInducible plasmid copy numberF’episomeAntibiotic resistanceElectrocompetent:efficiencyChemically competent:efficiency常规克隆及文库构建E. cloni® 10G (Classic)√√√√√ØØStr≥5 x109 106-109 E. cloni 10G (Elite)√√√√√ØØStr≥2 x1010 -E. cloni 10G (Supreme)√√√√√ØØStr≥4 x1010 -E.cloni 10G F’（Elite)√√√√√Ø√Str≥2 x1010 -E. cloni 5-alpha√√√ØØØØ--≥1 x108 不稳定的插入物或有毒基因Copycutter™ EPI400™√√√√√√ØStr≥1 x1010 ≥1 x107 大片段,plasmids和fosmids质粒TransforMax™ EC100™√Ø√√√ØØStr≥1 x1010 ≥5 x108 TransforMax EPI300™√Ø√√√aØStr≥1 x1010 ≥5 x108 TransforMax EPI300™-T1R√√√√√aØStr≥1 x1010 BAC克隆BAC-Optimised Replicator™ v2.0√Ø√√√aØAmp, Str≥1 x1010 -E. cloni BAC-Optimised 10G√Ø√√√ØØStr≥1 x1010 -BigEasy™-TSAb√√√√cØAmp, Str≥2 x1010 -噬菌体展示TG1√ØØØdØ√-≥4 x1010 -ER2738√√ØØdØ√Tet≥2 x1010 -SS320 (MC1061 F’)√ØØØeØ√Tet, Str≥4 x1010 -MC1061 F-ØØØØeØØStr≥4 x1010 -CRISPR sgRNA文库及慢病毒质粒克隆Endura™ØØfØgØØStr≥1 x1010 Site-directed mutagenesisCJ236ØØØØØØ√Cam≥1 x109 -Amp, ampicillin; Cam, chloramphenicol; Str, streptomycin; Tet, tetracyclinea: Requires OriV vectors (e.g., CopyRight® and pCCFOS) b: Requires IPTG c: Only with pJAZZ® vectors d: mcrB- only; not suitable for cloning eukaryotic genomic DNA e: mcrA- mcrB-; not suitable for cloning eukaryotic genomic DNA f: RecA13 only g: mcrB- mrr-; not suitable for cloning eukaryotic genomic DNAPrimary promoterBackground expression controlLow endotoxinAntibiotic resistanceElectrocompetent:efficiencyChemically competent:efficiency常规蛋白表达E. cloni EXPRESS BL21(DE3)T7Ø Ø -≥5x 109≥1x 107Hl-Control™ BL21(DE3)T7