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Lympho Spin Medium is a ready to use, sterile density gradient medium for the isolation of mononuclear cells (PBMC) in high yield from fresh peripheral whole blood and buffy coat by a simple centrifugation procedure. Mononuclear cells can be used for a wide range of downstream applications such as magnetic activated cell sorting (such as MACS¹) or stem cell research.

Lympho Spin Medium can be used as substitute for Ficoll™^,2 and Lymphoprep™^,3 without any need to change the existing protocols.

Separation scheme for PBMC enrichment with PBMC Spin Medium

At first carefully layer the sample material on top of the PBMC Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation aspirate the upper layer (Plasma and dilution buffer). Afterwards transfer the mononuclear cell layer to a new conical tube and wash the cells.

Fig. 1: Preparation of PBMC with Lympho Spin Medium. The sample material will be overlayed on top of density gradient medium.

Fig. 2.: Layers after density gradient centrifugation. The PBMC can be found on top of the Lympho Spin Medium. Erythrocytes, Granulocytes and dead cells will pass the medium and can be found at the bottom of the tube.

¹MACS is a trademark of Miltenyi Biotec GmbH; ²Ficoll™ is a trademark of GE Healthcare; ³Lymphoprep™ is a trademark of Axis-Shield / Alere

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