148资源库特色资源通用 HCP CHO 试剂盒的生成和开发视频观看视频特色资源抗体在研究 SARS-CoV-2 及其变体时的效用关注白皮书查看白皮书特色资源铁死亡途径文章查看文章协议技术提示白皮书文学文章视频科学海报加载更多资源类型协议 (25)技术提示 (20)白皮书 (4)文献 (27)文章 (35)视频 (17)科学海报 (20)

Alkaline Phosphatase Antibody Peroxidase Conjugated Properties Description Anti-ALKALINE PHOSPHATASE (Calf Intestine) (RABBIT) Antibody Peroxidase Conjugated - 200-4335-0100 Host Rabbit Target Species Bovine Known Cross Reactivity bovine Clonality Polyclonal Application ELISA : 1:2,000 - 1:10,000 Western Blot : 1:200 - 1:1,000 Immunohistochemistry: User Optimized Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format IgG Label Peroxidase (Horseradish) Concentration 1.0 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 100 µL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free GeneName ALP1 Relevant Links UniProtKB - P19111NCBI - P19111.2GeneID - 280993

Alkaline Phosphatase Antibody Peroxidase Conjugated Description

Background Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65°C inactivated most isoenzymes except Placental isoforms (PALP and SEAP). Synonyms rabbit anti-Alkaline Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody Immunogen Alkaline Phosphatase [Calf Intestine] Immunogen Type Native Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note Anti-Alkaline phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product. Purity/Specifity Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].  Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.