ADAM10 Antibody Properties Description Anti-ADAM10 (RABBIT) Antibody - 200-401-949 Host Rabbit Target Species Human Known Cross Reactivity human Clonality Polyclonal Application ELISA : 1:5,000 - 1:20,000 IF Microscopy : 1:250 - 1:1,000 Western Blot : 1:500 to 1:2000 Immunohistochemistry: 1:100-1:800 Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1.0 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName ADAM10 Relevant Links NCBI - 4557251UniProtKB - O14672GeneID - 102

ADAM10 Antibody Description

Background Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch, and its ligand delta, are all membrane-bound molecules, which are cleaved by proteases to release mature proteins or functional receptor.  ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta.  The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ) and MADM, respectively.  ADAM10 mRNA is expressed in a variety of human and bovine tissues. Anti-ADAM10 is ideal for investigators involved in NF-kappaB, Apoptosis, Cytokins and Growth factor research. Synonyms A disintegrin and metalloprotease domain 10 antibody, A Disintegrin And Metalloproteinase Domain 10 antibody, ADAM 10 antibody, ADAM metallopeptidase domain 10 antibody, CD 156c antibod, KUZ, MADM Immunogen This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ADAM10 protein. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note ADAM10 antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 84 kDa in size corresponding to ADAM10 protein by western blotting in the appropriate cell lysate or extract. Purity/Specifity ADAM10 IgG fraction antibody is directed against human ADAM10 protein.  The product was purified from monospecific antiserum by DEAE chromatography.  A BLAST analysis was used to suggest cross-reactivity with ADAM10 protein from human, bovine and rat based on 100% homology with the immunizing sequence.  Partial reactivity is expected against ADAM10 from mouse based on a single amino acid change relative to this sequence.  Reactivity against homologues from other sources is not known. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.