Apc11 Antibody Properties Description Anti-APC11 (C-terminal specific) (RABBIT) Antibody - 100-401-A15 Host Rabbit Target Species Human Known Cross Reactivity human Clonality Polyclonal Application ELISA : 1:2,000 - 1:10,000 Western Blot : 1:500 - 1:1,000 Immunohistochemistry: User Optimized Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format Antiserum Label Unconjugated Concentration 85 mg/mL by Refractometry Buffer None Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName ANAPC11 Relevant Links UniProtKB - Q9NYG5NCBI - 19924286GeneID - 51529

Apc11 Antibody Description

Background APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214.  APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex.  APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2.  APC11 shows both a cytoplasmic and nuclear localization.  APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte.  APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated. Synonyms rabbit anti-Apc11 Antibody, Anaphase promoting complex subunit 11 homolog antibody, APC11 anaphase promoting complex subunit 11 homolog antibody, Cyclosome subunit 11 antibody, Hepatocellular carcinoma associated RING finger protein antibody, HSPC 214 antibody, Apc Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note This antibody reacts with human APC11 by western blot and immunoprecipitation.  The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others).  Coimmunoprecipitation of related proteins (APC2) does occur.  A 9.8 kDa band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control.  Researchers should determine optimal titers for other applications. Purity/Specifity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources.  Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.