ARF-BP1 Antibody Properties Description Anti-ARF-BP1 (Rabbit) Antibody - 600-401-C03 Host Rabbit Target Species Human Known Cross Reactivity Human, mouse, rat Clonality Polyclonal Application ELISA : 1:10,000 - 1:40,000 IF Microscopy : 5.0 µg/mL Western Blot : 1.0 -2.0 µg/mL Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1.0 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName HUWE1 Relevant Links UniProtKB - Q7Z6Z7NCBI - NP_113584.3GeneID - 10075

ARF-BP1 Antibody Description

Background The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents. Anti-ARF-BP1 antibody is ideal for investigators involved in cancer research. Synonyms ARF-BP1, ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule; E3 ubiquitin-protein ligase HUWE1, KIAA0312, KIAA1578, UREB1 Immunogen ARF-BP1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ARF-BP1 protein. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note ARF-BP1 antibody has been tested in ELISA, WB, and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~481 kDa in size corresponding to ARF-BP1 by Western blotting in the appropriate cell lysate or extract. Daudi cell lysate can be used as positive control. Purity/Specifity Anti-ARF-BP1 Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with ARF-BP1 with Human, Rat and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with ARF-BP1 from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.