Acetyl Cholinesterase Antibody Properties Description Anti-ACETYL CHOLINESTERASE (Bovine Erythrocytes) (GOAT) Antibody - 200-101-232-0100 Host Goat Target Species Bovine Known Cross Reactivity bovine Clonality Polyclonal Application ELISA : 1:5,000 - 1:20,000 Western Blot : 1:500 - 1:5,000 ImmunoPrecipitation: 1:100 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format IgG Label Unconjugated Concentration 1.0 mg/ml by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 100 µL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName ACHE Relevant Links UniProtKB - P23795NCBI - P23795.2GeneID - 540446

Acetyl Cholinesterase Antibody Description

Synonyms goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE Immunogen Acetyl Cholinesterase [Bovine Erythrocytes] Immunogen Type Native Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note ACETYL CHOLINESTERASE Antibody has been assayed against 1.0 µg of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,200 to 1:5,000 of the reconstitution concentration is suggested for this product. Purity/Specifity Anti-ACETYL CHOLINESTERASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].  Cross reactivity against Acetyl Cholinesterase from other sources is unknown. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.