ScienCell™ Bovine Plasma Vitronectin is purified from bovine plasma by affinity chromatography. It is supplied as a sterile solution in Dulbeccos Phosphate-Buffered Saline (DPBS).
Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly.
Vitronectin is a glycoprotein found in plasma and extracellular matrix (ECM). It is a soluble, disulfide-linked dimer, composed of a 75 kDa and a 65 kDa peptide chain. In plasma, vitronectin is predominantly found as a single chain monomer [1]. The N-terminal of vitronectin contains multiple binding sites for a variety of structures. Vitronectin is involved in a number of biological functions including cell adhesion, spreading, migration, proliferation, extracellular anchoring, fibrinolysis, hemostasis, and complement immune defense [2, 3, 4]. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation, and differentiation.
ScienCell™ Bovine Plasma Vitronectin is purified from bovine plasma by affinity chromatography [5]. It is supplied as a sterile solution in Dulbeccos Phosphate-Buffered Saline (DPBS). Optimal conditions for cell attachment must be determined for each cell line and application.
Product Specification
Quantity: 100 µg
Concentration: 0.4 mg/ml
Storage buffer: DPBS, pH 7.4
Specifications:
| Catalog No. | 8538 |
| Country of Manufacture | United States |
| Product Code | BPV |
| Size/Quantity | 100 μg |
| Product Use | THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures. |
| Storage | It is recommended that the product be aliquoted and stored at -80°C. Vitronectin should be thawed slowly at 2-8°C with no agitation. Any precipitate that is present can be removed by centrifugation. Avoid repeated freeze/thaw cycles. |
| Shipping Info | Dry ice. |
| References | 1.Vuento M, Korkolainen M, Kuusela P, Holtta E. (1985) \"Isolation of a novel cell-attachment and spreading-promoting protein from human serum.\" Biochem J. 227: 421-7. 2.Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E. (1985) \"Vitronectin--a major cell attachment promoting protein in fetal bovine serum.\" Exp Cell Res. 160: 245-58.3.Akiyama KS. (1999) \"Purification of Vitronectin.\" Current Protocols in Cell Biology. 10.6.1-10.6.5 |
