PURO-1 is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. Puromycin terminates protein synthesis at the level of translation in prokaryotic and eukaryotic cells and is active against gram-positive bacteria and weakly active against gram-negative bacteria. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase (pac) gene [1, 2]. Exogenous expression of the pac gene is commonly used in conjunction with puromycin to generate stable cell lines.Most working concentrations range from 1-10 μg/ml; however, the optimal concentration should be determined for individual cell types and culture conditions. In most cases, puromycin kills 99% of non-resistant cells within 48 hours.

Specifications:

Catalog No.	0553
Country of Manufacture	United States
Product Code	PURO-1
Size/Quantity	10 mg/ml; 1 ml
Product Use	PURO-1 is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Storage	Store at -20°C; avoid freeze-thaw cycles.
Shipping Info	Dry ice.
References	[1] Vara J, Perez-Gonzalez JA, Jimenez A. (1985)\"Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase\". Biochemistry 24: 8074-8081. [2] Lacalle RA, Pulido D, Vara J, Zalacain M, Jimenez A. (1989) \"Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger\". Gene 79: 375-380.