This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF acts as a prosurvival factor and activates effector functions of granulocytes, monocytes/macrophages, and eosinophils. It promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells. Rat GM-CSF and mouse GM-CSF shares 69% amino acid sequence identity.

Specifications:

Catalog No.	142-03
Country of Manufacture	United States
Product Code	rrGM-CSF
Size/Quantity	5 μg
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store at -20°C after receiving. Upon Reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles.
Shipping Info	Dry ice
Synonyms	CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim
AA Sequence	MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK
Source	Escherichia coli
Molecular Weight	Approximately 14.5 kDa globular protein consisting of 128 amino acids residues.
Purity	>98% by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active. The ED50 is 1.0 x 10^8 units/mg, as determined by murine FDC-P1cell proliferation.
Physical Appearance	White lyophilized powder.
Formulation	Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin
Reconstitution	Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.