ScienCells GeneQuery™ Human cDNA Evaluation Kit, Deluxe (GQH-CED) assesses cDNA quality. The kit verifies successful reverse transcription of messenger RNA (mRNA) to complementary DNA (cDNA), reveals the presence of genomic DNA (gDNA) contamination in cDNA samples, and detects qPCR inhibitor contamination. Good quality cDNA is a critical component for successful gene expression profiling. The GQH-CED kit is highly recommended for cDNA applications such as GeneQuery™ qPCR arrays.
Each primer set included in GQH-CED qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.
Quality Control
Each primer set is validated by qPCR melt curve and amplification curve analyses. The PCR products are analyzed by gel electrophoresis to confirm single band amplification.
Specifications:
| Catalog No. | GK991 |
| Country of Manufacture | United States |
| Product Code | GQH-CED |
| Size/Quantity | 100 Reactions |
| Product Use | GQH-CED is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | This product is shipped at ambient temperature. Upon receipt, the vials should be stored at 4°C and are good for up to 12 months. For long-term storage (>1 year), store the vials at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
