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LI-COR/IRDye® 680RD Streptavidin/[{size: 0.5 mg, price_type: Default, price_USD: 135.0, price_GBP: 152.0, long_desc:
IRDye 680RD Streptavidin can be used as a secondary detection reagent for protein arrays, microscopy, In-Gel Westerns, a
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产品说明
IRDye 680RD Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 µg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.Recommended DilutionsApplicationSuggested RangeTween 20*SDS*Odyssey® Western blot detection1:1,000 - 1:5,0000.1 - 0.2% (v/v)0.02 - 0.1% (v/v)OtherUser optimizedUser optimizedUser optimizedOptimum dilutions will vary and should be determined empirically.* Added to reduce non-specific background staining.See the complete line of IRDye Streptavidins.
美国LI-COR公司成立于1971年,其产品广泛应用于全球的100多个国家,已成为从事植物生理及生态学研究的科学家的首选仪器。LI-COR公司的生态仪器应用于农学、生物技术、植物学、生态学、林业、园艺、湖沼生物学、气象学、海洋学、光学研究、植物生理学和日光研究等不同学科。主要产品有光合作用测定系统(LI-6400)、CO2/H2O气体分析系统(LI-7000/LI-7500)、CO2气体分析系统(LI-820)、稳态气孔计(LI-1600)、叶面积仪(LI-3100/LI-3000A)、植物树冠分析仪(LAI-2000)、露点发生器(LI-610)、数据采集器(LI-1400)、光强表(LI-250)、气象站(LI-1401/LI-1405)和各种光量子传感器等。
LI-COR公司的产品正是国际生态学研究的最佳仪器,无论是研究成果的发表,还是应用,使用LI-COR产品与否,已体现到其结果的可靠性和真实性。有人对国际著名生态学杂志作过粗略统计,凡涉及到使用LI-COR产品或其同类厂家仪器的文章中,以使用LI-COR产品的文章最多,约占70%以上。
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