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Hampton research/Silver Bullets/Silver Bullets/0.5 ml, Deep Well block format/HR2-096

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HR2-096

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Applications Features DescriptionSilver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.Silver Bullets reagent portfolio •Organic salts and acids •Biologically active small molecules •Amino acids and peptides •Macromolecular digestsThe Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to: •Stabilize the conformation of the protein •Perturb the interaction of the protein with the solvent •Participate in forming important lattice contacts •Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystalThe Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 - 0.7 mL at Room Temperature 0.03 - 0.55 mL at -20ďż˝C, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.Silver Bullets reagent volume is 0.5 ml (each well).ThermoFluor is a registered trademark of Johnson & Johnson. Show More

美国Hampton Research公司是蛋白质晶体研究方面的专家，是一个从事蛋白质晶体研究研制的专业公司，为全球科研人员提供晶体研究工具和试剂，完整的晶体培养体系；同时提供晶体设计和合成方面的服务。其提供的试剂长期以来在该领域居于领先地位，为广大研究人员提供了极大的方便，促进了蛋白质晶体的研究，为世界范围内的蛋白质晶体研究人员所信赖。