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Zyagen/AES Antibody/4050/1 Ea

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4050

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APO-3607 Background AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin. Description Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 μg/mL. Immunocytochemistry of AES in HeLa cells with AES antibody at 10 μg/mL. Specifications Additional Names AES Antibody: Amino-terminal enhancer of split, GRG, ESP1, TLE5 User Note Optimal dilutions for each application to be determined by the researcher. Isotype IgG Disclaimer This product is for research use only. Formulation Physical State: Liquid Concentration: 1 mg/mL Conjugate None Clonality/Clone This is a polyclonal AES Antibody. Purification AES Antibody is affinity chromatography purified via peptide column. Host AES antibody was raised in rabbit. Please use anti-rabbit secondary antibodies. Immunogen AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. Application AES antibody can be used for the detection of AES by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. Tested Application E, WB, ICC, IF Buffer AES Antibody is supplied in PBS containing 0.02% sodium azide. Blocking Peptide Cat. No. ZP-3607 - AES Peptide Storage AES antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Species Reactivity H, M, R Protein GI Number 39812019 Protein Accession Number NP_945320 Reference Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57. Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52. Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16. Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.]

产品简介

我公司全国总代理,华东地区代理zyagen laboratories专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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关键词:zyagen laboratories
简介：我公司全国总代理,华东地区代理zyagen laboratories专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
全国总代理,华东地区代理产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞疗、临床应用等领域。全国总代理,华东地区代理范围内免费运输，如出现运输质量问题，我们可以包退换货。完善的库存及供应体系以及高效稳定的纯化技术，保证产品均能现货供应和产品质量的稳定性。
品牌名称：
【生物分子】抗生素/抗真菌素维生素氨基酸核苷酸脂糖类白三烯前列腺素药物结合物抗氧化剂药理活性化合物类固醇激素结合物半抗原反应半抗原载体结合物放射性核素血小板活化素 tRNA 活性染料和化合物亲和素/链霉亲和素
【蛋白质/抗原/多肽】免疫球蛋白封闭肽人蛋白和抗原小鼠蛋白和抗原细菌蛋白和抗原病毒蛋白和抗原植物蛋白和抗原其它蛋白和抗原细胞质蛋白总蛋白膜蛋白核蛋白细胞裂解和提取物线粒体蛋白细胞裂解组织提取重组细胞因子
【常用生化试剂】EDTA DTT Tris SDS MOPS HEPES 水分子生物学缓冲液蛋白生化缓冲液去垢剂染色试剂溶剂酸碱化学试剂其它生化试剂
【PCR/RT-PCR/qPCR】PCR试剂 PCR对照特异性PCR试剂盒 PCR克隆试剂盒 RNA 载体及构建 PCR克隆载体 M13克隆载体细菌克隆载体文库及构建 cDNA文库基因组文库噬菌体展示文库
【酶】限制性内切酶蛋白酶核酸酶激酶聚合酶连接酶
【cDNA及合成纯化】cDNA全长基因 RNA cDNA合成 cDNA相关试剂
【核酸/蛋白合成】Oligo纯化核酸合成柱核酸合成试剂
【克隆与表达】克隆基因克隆试剂盒克隆筛选
【表达分析】 Northern印迹分析分支DNA和mRNA定量
【蛋白分析】 PAGE凝胶制备蛋白电泳试剂预制蛋白凝胶
【核酸纯化】 DNA凝胶纯化 DNA提取纯化 PCR产物纯化
【RNAi技术】 siRNA 反义寡核苷酸 RNAi定量 RNAi文库
【蛋白检测】 Western印迹报告基因检测蛋白检测试剂盒
【实验动物】转基因动物小鼠大鼠模式动物
【临床检测试剂】生化检测遗传学检测血液检测
【相关检验试剂】食品安全检测药品安全检测
【蛋白纯化】蛋白提取蛋白透析蛋白定量蛋白稳定
【细胞培养】血清血清替代物细胞培养基细胞细胞株感受态细胞新鲜细胞分离
【干细胞】干细胞/祖细胞原代细胞干细胞培养基
【蛋白修饰】蛋白标记载体蛋白结合其它
【细胞生物学检测】细胞凋亡细胞分离细胞增殖
【药物筛选】荧光素细胞活力/增殖/毒性检测
【免疫检测】放射性免疫检测化学发光免疫检测