Rabbit MonoclonalRevmab/Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193; Histone H3, K36M Mutant (50ug)/31-1085-00/1 Ea RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-... ProductDetailsProductNameAnti-HistoneH3K36MRabbitMonoclonalAntibody[RM193]ProductDescriptionRabbitmonoclonaltoHistoneH3K36M;HistoneH3,K36MMutantCatalogNo.31-1085-00CloneNameRM193ProductDataSheetAnti-HistoneH3K36MRabbitMonoclonalAntibodyDataSheetRM193SpecificityRM193reactstotheHistoneH3K36Mmutant.NocrossreactivitywithwildtypeHistoneH3.ImmunogenApeptidecorrespondingtoHistoneH3K36MmutantApplicationWesternBlotELISAImmunohistochemistryImmunocytochemistryChromatinIPSpeciesReactivityAllConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size50ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.5ug/mL–2ug/mL;ELISA:0.5ug/mL-2ug/mL;Immunohistochemistry(IHC):0.5–5ug/mL;Immunocytochemistry(ICC/IF):0.5–5ug/mL.KeywordsH3F3A;H3F3B;K36M;bonetumour;chondroblastomaReferencesforHistoneH3K36MMutantantibody[RM193]AmaryMFetal.TheH3F3K36MmutantantibodyisasensitiveandspecificMarkerforthediagnosisofchondroblastoma.Histopathology10.1111/his.12945(2016).IHC-P;Human.Readmore(PubMed:26844533)D.Fangetal.ThehistoneH3.3K36MmutationreprogramstheepigenomeofchondroblastomasScience10.1126/science.aae0065(2016).ChIP;Human.Readmore(PubMed:27229140).ProductImages

WesternBlotanalysisofcelllysatespreparedfrom293T transfected with a DNA construct encodingwild type or K36M mutant proteins of Histone H3.1 and H3.3, using anti-Histone H3 K36M clone RM193.

Immunohistochemicalstainingofformalinfixedand paraffin embedded 293T cells transfected with a DNAconstruct encoding Histone H3 K36M, stained with anti-Histone H3 K36M clone RM193.

Immunohistochemicalstainingofformalinfixedparaffin embedded (FFPE) Chondroblastoma tumorcell tissue sections.

ImagecourtesyofAdrienneFlanagan,Department of Histopathology, RONH, Stanmore, Middlesex, UK

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.