31-1053-00,RevMAb Biosciences,Rabbit Monoclonal,RevMAb Biosciences,+,兔单克隆,Array.Array.Array蚂蚁淘厂家直采.
Rabbit MonoclonalRevmab/Anti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM157; H3K79me3, Histone H3 (trimethyl K79)/31-105 RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-... ProductDetailsProductNameAnti-Trimethyl-HistoneH3(Lys79)RabbitMonoclonalAntibody[RM157]ProductDescriptionRabbitmonoclonaltoTrimethylatedHistoneH3Lysine79,H3K79me3,HistoneH3(trimethylK79)CatalogNo.31-1053-00CloneNameRM157ProductDataSheetAnti-Trimethyl-HistoneH3(Lys79)RabbitMonoclonalAntibodyDataSheetRM157SpecificityRM157reactstoHistoneH3trimethylatedatLysine79(K79me3).NocrossreactivitywithmonomethylatedLysine79(K79me1),dimethylatedLysine79(K79me3),orothermethylationsinhistoneH3.ImmunogenAtrimethyl-peptidecorrespondingtoTrimethyl-HistoneH3(Lys79).ApplicationWesternBlot(WB)
ELISAMultiplexSpeciesReactivityHumanVertebratesConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.2ug/mL–1ug/mL;ELISA:0.1ug/mL-0.5ug/mL;Multiplex:0.1ug/mL–0.5ug/mL.Keywords.ProductImages
![\"A\"](\"http://www.baidu.com/images/revmab/Fig-1_RM157e1-300x300.png\")
APeptidedotblotshowsRM157onlyreactstoHistone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity. with nonmodified Lysine 79 (H3K79Ctrl), monomethylated. Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).![\"RM157\"](\"http://www.baidu.com/images/revmab/Fig-2_RM157_e1-300x300.png\")
RM157specificallyreactstoHistoneH3trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.![\"Western\"](\"http://www.baidu.com/images/revmab/Fig-3_RM157_e1-300x300.png\")
WesternBlotofrecombinanthistoneH3.3(1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells.
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.
