Agrisera/Donkey anti-Goat IgG (H&L), DyLight® 633 conjugated/AS12 2131/
| | product information | | Background | | Donkey anti-Goat IgG (H&L) - DyLight® 633 Conjugatedis a secondary antibody conjugated to DyLight® 633, which binds togoatIgG(H&L)in immunological assays. DyLight®633 has Amax =638 nm, Emax =658 nm. Antibodies are are affinity purified using solid phasegoat IgG. DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. | | Immunogen | | Purified goat IgG, whole molecule | | Host | | Donkey | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified donkey IgG | | Format | | Lyophilized | | Quantity | | 1 mg | | Reconstitution | | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. | | Storage | | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | Tested applications | | | | Related products | | other dylight anti-goat secondary antibodies | | Additional information | | Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. | |
| application information |
| Recommended dilution | | 1:20- 1:2,000 for most applications |
| Expected | apparent MW | | |
| Confirmed reactivity | | |
| Predicted reactivity | | |
| Not reactive in | | |
| Additional information | | Based on immunoelectrophoresis, this antibody reacts with: chains on goat IgG light chains on all goat immunoglobulins. No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. |
| Selected references | | to be added when available |
