Agrisera/Goat anti-Human IgG (H&L), HRP conjugated/AS10 764/
| | product information | | Background | | Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays. | | Immunogen | | Purified human IgG | | Host | | Goat | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified goat IgG | | Format | | Lyophilized | | Quantity | | 1 mg | | Reconstitution | | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. | | Storage | | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | Tested applications | | ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC) | | Related products | | antibodies to human immunoglobulins | | Additional information | | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase | |
| application information |
| Recommended dilution | | optimal working dilution should be determined by the investigator |
| Expected | apparent MW | | |
| Confirmed reactivity | | human IgG (H&L) |
| Predicted reactivity | | human IgG (H&L) |
| Not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| Additional information | | This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis. No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis. |
| Selected references | | to be added when available |
