Agrisera/Goat anti-Human kappa chain, ALP conjugated, min. cross-reactivity to Mouse serum/AS10 1410
| | product information | | Background | | Goat anti-human kappa (k) chain is a secondary antibody conjugated to ALP (alkaline phosphatase), which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. | | Immunogen | | Purified human Kappa Chain BAA37169.1 | | Host | | Goat | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified IgG | | Format | | Liquid | | Quantity | | 0.5 mg | | Reconstitution | | | | Storage | | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | Tested applications | | | | Related products | | other ALP (alkaline phosphatase) conjugated secondary antibodies against human immunoglobulins | | Additional information | | Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth. Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins,heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins,mouse serum proteins. | |
| application information |
| Recommended dilution | | This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for Immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot |
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