Agrisera/Goat anti-Human IgG (H&L), F(ab)2 fragment, ALP conjugated/AS10 774/
| | product information | | Background | | Goat anti-human IgG (H&L), F(ab)2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays. | | Immunogen | | Purified human IgG | | Host | | Goat | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified goat IgG, F(ab)2 fragment | | Format | | Liquid | | Quantity | | 0.5 mg | | Reconstitution | | | | Storage | | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | Tested applications | | ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC) | | Related products | | other secondary antibodies to human immunoglobulins | | Additional information | | ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative. Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG. | |
| application information |
| Recommended dilution | | 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC) |
| Expected | apparent MW | | |
| Confirmed reactivity | | human IgG (H&L) |
| Predicted reactivity | | human IgG (H&L) |
| Not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| Additional information | | This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis. |
| Selected references | | to be added when available |
