Agrisera/AtpC | Gamma subunit of ATP synthase, chloroplastic/AS08 312/-蚂蚁淘生物


product information
Background		ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Immunogen		synthetic peptides derived from Arabidopsis thalina chloroplast localized ATP synthase subunit gamma chain 1 and 2 protein sequence ( At4g04640 and At1g15700) and Chlamydomonas reinhardtii ATP synthase subunit gamma protein sequence ( A8HXL8) coupled to KLH
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100 µl
Reconstitution		For reconstitution add 100 µl of sterile water
Storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS08 370 \| anti-ATP synthase, whole enzyme AS08 304 \| anti-ATP synthase subunit alpha antibody AS03 030 \| anti-ATP synthase subunit beta hen antibody AS05 071 \| anti-ATP synthase subunit c antibody AS07 260 \| H+ATPase \| plasma membrane H+ATPase Plant protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1: 10 000 (WB), 1: 1000 (ELISA)
Expected \| apparent MW		35.3 \| 42 (Chlamydomonas reinhardtii) 35.6 \| 38 (Spinacia oleracea)
Confirmed reactivity		Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity		Glycine max, Lens culinaris, Nicotiana tabacum, Physcomitrella patens, Pisum sativum, Populus jackii, Vitis vinifera, cyanobacteria
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information		apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989)
Selected references		Naranjo et al. (2015). The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis. Plant Cell Environ. 2015 Oct 17. doi: 10.1111/pce.12652.Dwyer et al. (2012). Antisense reductions in the PsbO protein of photosystem II leads to decreased quantum yield but similar maximal photosynthetic rates. J. Ex. Bot. 63(13):4781-95.

application example

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit gamma deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), Chlamydomonas reinhardtii thylakoid membrane preparation (4) were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS08 312, 1: 25 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare), exposure time 30’’ and 3 min (overexposed).

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet

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