Agrisera/RPL1 | 50S ribosomal protein L1/AS11 1738/-蚂蚁淘生物


product information
Background		Rpl1 (50S ribosomal protein L1) belongs to the ribosomal protein L1P family. This protein binds directly to 23S rRNA, acts also as a translational repressor protein.
Immunogen		KLH-conjugated synthetic peptide derived from all known cyanobacterial Rpl1 sequences including Synechocystis sp. 6803, P36236
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100 µl
Reconstitution		For reconstitution add 100 µl of sterile water.
Storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		western blot (WB)
Related products		AS13 2650 \| anti-L13-1 \| 60S ribosomal protein L13-1, rabbit antibodies AS09 478\| anti-L13-1 \| 60S ribosomal protein L13-1, rabbit antibodies AS08 331\| anti-L-30 \| 50S ribosomal protein L30, rabbit antibodiesAS15 2876 \| anti-RPL2 \| ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 \| anti-RPL33 \| 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 \| anti-RPL36 \| 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 \| anti-RPL37 \| ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 \| anti-RPS1 \| ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 \| anti-RPS12 \| ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 \| anti-RPS15 \| 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS10 719 \| anti-Rps2 \| anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS08 309 \| anti-S1 \| anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 \| anti-S14 \| 40S ribosomal protein S14-1 , rabbit antibodiesAS15 3068 \| anti-S4 \| mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 \| anti-S10 \| mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 \| anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodies Algal protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1 : 2000 with standard ECL (WB)
Expected \| apparent MW		25 kDa
Confirmed reactivity		Anabaena sp., Synechococcus sp. 7942, Synechocystis sp. 6803
Predicted reactivity		all known cyanobacterial species
Not reactive in		E.coli
Additional information		to be added when available
Selected references		Linhartová et al. (2014). Accumulation of the Type IV prepilin triggers degradation of SecY and YidC and inhibits synthesis of Photosystem II proteins in the cyanobacterium Synechocystis PCC 6803. Mol Microbiol. 2014 Jul 24. doi: 10.1111/mmi.12730.

application example

$\"western$

5 µg of total protein from Anabaena sp. (1), Synechococcus sp. 7942 (2), Synechocystis sp. 6803 (3) extracted with Agrisera protein extraction buffer PEB were separated on 4-12% NuPAGE and blotted 1h to PVDF. Blots were blocked with ECL Advance Blocking Reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Healthcare). Exposure time was 180 seconds.

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