Agrisera/ATG7 | Autophagy-related protein 7/AS13 2699/-蚂蚁淘生物


product information
Background		Autophagy-related protein 7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1. Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
Immunogen		A synthetic peptide corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100 µl
Reconstitution		For reconstitution please add 100 µl of sterile water
Storage		After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Tested applications		Immunohistochemistry (IHC), immunofluorescence (IF)
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Additional information

application information
Recommended dilution		A dilution of 1:200 to 1:3000 dilution is recommended for these applications. Optimal dilutions/concentrations should be determined by the end user.
Expected \| apparent MW
Confirmed reactivity		human, rat
Predicted reactivity		not yet tested in other species
Not reactive in
Additional information		to be added when available
Selected references		to be added when available

Application example

Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (AS13 2699) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.

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