product information Background The PsbN protein is chloroplast-encoded, low molecular weight protein annotated as a photosystem II subunit however it seems that it is not a constituent subunit of PSII but is required for PSII repair from photoinhibition.  Immunogen KLH-conjugated synthetic peptide chosen from PsbN protein of Arabidopsis thaliana Uniprot: P62113, TAIR: AtCg00700 Host Rabbit Clonality Polyclonal Clone Purity Serum Format Lyophilized Quantity 50 µl Reconstitution For reconstitution add 50 µl of sterile water. Storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications western blot (WB) Related products collection of antibodies to PSII proteins Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 5000 with standard ECL (WB)
Expected | apparent MW		4.7 kDa
Confirmed reactivity		Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity		Arabis alpina, Camelia sp., Canna indica, Costus pulverulentus, Glycine max, Helianthus tuberosus, Hordeum vulgare, Lactuca sativa, Lilium sp., Manihot esculenta, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Populus trichocarpa, Saccharum officinarum, Solanum tuberosum, Sorghum timorense, Spinacia oleracea, Tricitum aestivum, Thaumatococcus daniellii, Vitis vinifera
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information		to be added when available
Selected references		Torabi et al. (2014). PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum. Plant Cell. 2014 Mar;26(3):1183-99. doi: 10.1105/tpc.113.120444. Epub 2014 Mar 11.

application example 5 µg of membrane proteins from Nicotiana tabacum WT and Δ psbN mutant isolated with homogenization buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA, 2 mM EGTA, 10 mM DTT ) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 3% BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 12h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:25 000 in BSA for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 53 seconds. Courtesy of Dr. Jörg Meurer, Biozentrum der Ludwig-Maximilians-Universität München, Germany

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