Anatrace/ProteoSEC Size Exclusion Column/ SEC-16/60-6-600/ 1 Column
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Gel filtration is a proven technique which is widely used for size-based molecular separation. ProteoSEC Gel Filtration resin is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Pre-packed ProteoSEC Gel Filtration Resin is available in 2 column formats: 16/60 and 26/60.ProteoSEC Gel Filtration Resin has a balanced design formulated to offer high recovery and high selectivity according to individual applications. The key benefits are:· High resolution· High selectivity· High recovery· Highly scalable· Excellent physical and mechanical stability· Cost effectiveThe base matrix is a highly cross-linked polysaccharidecomposite resin and is very stable in the presence of mostaqueous solutions and organic solvents.ProteoSECGel Filtration Resin Selection Guide:
| | ProteoSEC 3-70 HR | ProteoSEC 6-600 HR |
| Separation range: | 3 kDa – 70 kDa | 6 kDa – 600 kDa |
| Column dimensions: | 16 mm ID x 60 cm column length (120 ml CV) 26 mm ID x 60 cm column length (320 ml CV) |
| Particle size: | 20 – 50 µm (35 µm avg.) | 20 – 50 µm (35 µm avg.) |
| Format supplied: | Pre-packed 16/60 and 26/60 ProteoSEC columns |
ProteoSECGel Filtration Column Specifications: | ProteoSEC 3-70 HR and ProteoSEC 6-600 HR |
| Matrix: | Highly cross-linked polysaccharide composite of dextran and agarose |
| Particle size (µm): | 35 µm (in the range of 25-50 µm) |
| Column body max pressure (*): | 6 Bar, 0.6 MPa (3-70 columns) and 5 Bar, 0.5 MPa (6-600 columns) |
| Operating flow velocity: | 10-50 cm/hour: 0.3 - 1.6 ml/minute (16/60 columns) 0.8 – 4.4 ml/minute (26/60 columns) |
| pH stability: | 2 - 14 (short-term) and 3 – 12 (long-term) |
| Working temperature: | +4°C - +30°C |
| Chemical stability: | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6 M guanidine hydrochloride, 8 M urea, 30% isopropanol, 20% ethanol (concentration of alcohol should not exceed 30% v/v) |
| Avoid: | Oxidizing agents |
| Storage: | 0.02% sodium azide or 20% ethanol |
| *Maximum resin pressure for all the 3 – 70 columns is 4 Bar. Maximum resin pressure for all the 6 – 600 columns is 3 Bar. |
