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确定 MolecularFormula:C22H40O11CASNumber:181135-57-9FormulaWeight:480.5CMC:(H2O)~7.6mM(2) (0.37%)CMC:(0.15MNaCl)~7.3mM(2)AggregationNumber:(H2O)(2)~25dn/dc:0.1317ml/gPurity:≥99%β+αbyHPLCanalysisPercentAnomer:
Anatrace全球公认的膜蛋白研究用产品供应商,为膜蛋白提取与纯化提供高纯度生化产品,包括各种表面活性剂、去垢剂、试剂盒及各种研究用工具书等。Anatrace公司成立于1984年,是USB Corporation的全资附属子公司。2008年1月,Anatrace和USB Corporation一起被合并到美国Affymetrix旗下。重组后的Anatrace一直坚持公司的发展宗旨,致力于生产和供应独一无二的、高纯度的去垢剂和合成脂,成为全球公认的膜蛋白研究试剂领先型生产商。公司产品:DDM , Dodecyl MaltosideDM, Decyl MaltosideOG, Octyl GlucosideLM NG, Lauryl Maltose Neopentyl GlycolLDAOCYMAL-5
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MethodsfortheDetectionofD-Amino-AcidOxidaseRyuichiKonno1*1DepartmentofMicroBIOLOGy.DokkyoUniversitySchoolofMedicine,Mibu,Tochigi321-0293.Japan.*Towhomcorrespondenceshouldbeaddressed:RyuichiKonno,DepartmentofMicrobiology.DokkyoUniversitySchoolofMedicine,Mibu,Tochigi321-0293.Japan.Email:konno@dokkyomed.ac.jpBiol.Proced.Online1998;1:27-31.doi:10.1251/ 查看更多


GlucosamineRapidAssayMRTHOD:Placesample(containing0.5-10 gGlcN)inaPyrexscrewcappedtube.AddHCltoafinalconcentrationof2Nandafinalvolumeof0.6ml.Flushwithnitrogen.Stoppertightlyandhydrolyze16hat100oC.Preparestandardscontaining0-20 gGlcNfromastocksolutionof1.5mg/ml.(Use0,3,5,8,10,12mlofstocksolutionandaddwatertomakeupto300 l.Thenadd300 查看更多


EnzymeKineticsassayoftheWTToassay17b-HSDactivityinlysates,cellswereharvested48haftertransfectionusingPBSEnzymeFreeCellDissociationSolution(specialtyMediaInc.,Lavellette,NJ),frozenondryiceandstoredat-80C.Cellpelletswerethawedonice,resUSPendedin:*10mMTris-HCl(pH7.4)/150mMKCL/1mMEDTA/2mMDithiothreitol(DTT)atproteinconcentrationof3-6mg/ml,andsnap-froze 查看更多


CellulaseassaysCellulaseenzymesshowactivityduringtheripeningofsomefruits,wheretheireffectsoncellwallsresultsinsofteningofthefruit.Incasesofprogrammedcelldeath,suchastheformationofaerenchyma(largeairspacesinthecortexofplantsinfloodedsoils),andintheabscissionzonesofleavesandfruits,cellulasesareonceagainveryactive,breakingdownthecellulosewallsofthedea 查看更多


ProteaseassayIncertainfruits,suchaspineapplesandmangoes,thefleshcontainsprotein-digestingenzymes(proteases).Thesemayplayapartinhelpingtosoftenthefruittissuesasthefruitripens,makingitevenmoreattractivetoanimalsthatmightdispersetheseeds.Soperhapstheactivitiesoftheseproteasesenzymeswillincreaseduringtheripeningprocess.Applicationsofplantproteases,such 查看更多


Polyphenoloxidase(catecholoxidases)assayBrowningofthecutsurfaceofsomefruitsandvegetablesisduethepresenceofagroupofenzymescalledpolyphenoloxidases.Theseenzymesarereleasedbythebrokencellsandtheycatalysethereactionbetweencolourlessmoleculescalledpolyphenolsandmolecularoxygen.Thisreactioncreatescolouredcompoundsandthesenewcompundscanspontaenouslycrossr 查看更多


PolygalacturonaseassayThisenzymeisfamousforbeinginvolvedinthedevelopmentoftheGMOtomatoes(moreinformationfromthelinkatthefootofthispage).Thecellsofthesetomatoeshavebeengeneticallymodifiedtocontainareversedcopyofthegeneforthisenzyme.Thisproducesanti-sensemRNAwhichcombineswiththenormalmRNAforpolygalacturonase,effectivelysupressingitssynthesis.Asaresul 查看更多


PectinaseassayPectinasesareactuallyamixtureofenzymes,which,alongwithotherssuchascellulase,arewidelyusedinthefruitjuiceindustrywheretheyarewidelyusedtohelpextract,clarifyandmodifyfruitjuices.(Seethefootofthispageformoreinformation)Pectinsarelargepolysaccharidemolecules,madeup(mainly)ofchainsofseveralhundredgalacturonicacidresidues.Enzymesinthispecti 查看更多




