OTHER TOOLS AND RESOURCES

CHEMICAL STRUCTURE

3,5-difluoro-4-hydroxybenzylidene imidazolinone-2-oxime (DHFO)

MOLECULAR FORMULA:

C₁₂H₈F₂N₃O₃

MOLECULAR WEIGHT:

281.2

CAS NAME/NUMBER:

(E)-4-((Z)-3,5-difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime/

FLUORESCENCE SPECTRA

A. Absorption and fluorescence emission spectra of Corn^TM/DFHO in pH 7.4 buffer.B.Absorption and fluorescence emission spectra of Broccoli^TM/DFHO in pH 7.4 buffer.

PRESENTATION:Lyophilized dye

QUALITY ASSURANCE:Products are analyzed by¹H NMR and LC-MS and provided at purity of >95% by HPLC.

USAGE STATEMENT:This product is intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Due to the highly specific nature of fluorophores and aptamers, we cannot predict or be held responsible with respect to how Lucerna^TMproducts will behave in its customers systems. Researchers using Lucerna^TMproducts should conduct optimization studies to achieve the optimal result possible for their intended application.

DFHO IMAGES

A.Live-cell imaging of HEK293T cells expressing U6 promoter driven Corn^TMin the presence of 10 µM DFHO. Images are Hoechst-stained nuclei (blue) and Corn^TMfluorescence (yellow), individually or overlayed. Images were acquired using YFP and DAPI filter sets.B.Live-cell imaging of E. coli cells expressing tRNA promoter driven Corn^TMin the presence of 10 µM DFHO.

REFERENCE FOR THE PRODUCT:

Song W, Filonov GS, Kim H, Hirsch M, Li X, Moon JD, Jaffrey SR. 2017.Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex.Nat Chem Biol.

THIS PRODUCT WAS CITED IN:

LICENSING:

Lucerna has the exclusive rights to all patents covering the use of this product. All commercial users should contact Lucerna for a use license.