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MatTek/EpiCorneal™/epicorneal/1 Ea

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TheEpiCorneal3Dhumantissuemodelprovidesahighlypredictivenon-animalalternativetoassessdrugdelivery,infection,woundhealing/tissueregenerationanddiseasemodeling(i.e.DryEye).Technology:MatTek’sEpiCornealtissuemodelconsistsofnormalhumancornealepithelialcellsculturedtoformastratified,squamousepitheliumwhichcloselyparallelsnormalhumancornealtissue.Thecornealcells,culturedonspeciallypreparedcellcultureinsertswithserum-freemedium,differentiatetoformamulti-layeredstructure.TEMAnalysisofEpiCornealillustratesthepresenceof1)microvilli,2)tightjunctions(whitearrows),and3)desmosomes(blackarrows)TightJunctionProteinZO-1(green)Usedindrugdeliveryanddrugdevelopment,EpiCornealisamitoticallyandmetabolicallyactivetissuewithbarrierpropertiesapproachingthoseofthehumancornea.EpiCornealcanbestimulatedtoreleasemanycytokinesknowntobeimportantininflammationanddiseasessuchasdryeye.Further,theadvanced3Dtissuemodelexpressescorneaspecificdrugtransportersandmetabolizingenzymes.BarrierPropertiesofEpiCornealcomparedtoEpiOcularmeasuredbytransepithelialelectricalresistance(TEER).GeneExpressionAnalysisshowstheexpressionofcorneaspecificMarkers,drugmetabolizingenzymesandtransportersincludingAldehydeDehydrogenase(ALDH3A1),PyrimidineNucleotideOxidoreductase(TRXR1)andMucin4(MUC4)Applications:CornealDrugDeliveryandCornealToxicityEpiCornealcloselyrecapitulatesthephysiology,3Dtissuearchitecture,andfunctionofthehumancorneaforuseinpharmaceuticaldevelopmentapplications.ContactDr.YuliaKaluzhnyformoreinformation.BrowseourreferencelibrarytoseehowourEpiCornealtissuehasbeenusedintheseareasofstudy.TechSpecs:TissueKit:AstandardEpiCornealkit(COR-100)consistsof24tissues.(Tissue“kits”containtissues,asmallamountofculturemedium,andplasticware;contactMatTekforspecifickitcontents)Formats:9mmindividualinserts–tissueculturesubstrateischemicallymodifiedwithaporesizeof0.4μmCulture:Air-liquidinterfaceHistology:4-5celllayers–stratified,squamousmorphologyandtissuestructureLotnumbers:Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualkitswithinagivenlotoftissues.Alltissuekitswithinalotareidenticalinregardstocells,medium,handling,cultureconditions,etc.Shipment:At4°Conmedium-supplemented,agarosegelsShipmentday:EveryMondayDelivery:TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-ThursdaydependingonlocationShelflife:Includingtimeintransit,tissuesmaybestoredat4°Cforupto3dayspriortouse.However,extendedstorageperiodsarenotrecommendedunlessnecessary.Inaddition,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning)CellsType:Normalhumancornealepithelialcells(HCEC)Geneticmake-up:SingledonorDerivedfrom:HealthydonorScreenedfor:HIV,Hepatitis-B,Hepatitis-C,mycoplasmaMediumBasemedium:Dulbecco’sModifiedEagle’sMedium(DMEM).Growthfactors/hormones:Epidermalgrowthfactor,insulin,hydrocortisoneandotherproprietarystimulatorsofcornealepithelialdifferentiation.Serum:None.Antibiotics:Penicillin(50U/ml)/Streptomycin(50µg/ml)Anti-fungalagent:AmphotericinB(0.125µg/ml).pHIndicator:Phenolred.Alternatives:Phenolred-free,antibiotic/anti-fungal-free,orhydrocortisone-freemediumandtissueareavailable.Agentsareremovedatleast3dayspriortoshipment.Maintenancemedium:MostexperimentswithEpiCorneal™areperformedwithin48hours.However,forlongerexperiments,thetissuecanbemaintainedinCOR-100-MM(identicaltoEpiCorneal™assaymedium).QualityControlandSterilityVisualinspection:Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipmentSterility:Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontaminationScreeningforpathogens:AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“BiosafetyinmicroBIOLOGicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalserviceNotificationoflotfailure:IfatissuelotfailsourQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutchargewhenappropriate.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossIBLe(Undernormalcircumstances,ET-50failureswillbenotifiedbyWednesday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)

MatTek公司是麻省理工学院两个化学工程教授于1985成立的科技公司。1991年，该公司利用其核心的聚合物表面改性技术进入新兴的组织工程领域，目前已成长为的组织工程技术公司，致力于生产创新性的3D重构人体组织模型，该模型相较于传统2D细胞模型具有更可靠的实验结果。其生产的重构皮肤，眼部和呼吸道组织模型已广泛应用于化妆品，化工，医药和家用产品等行业的毒理学监控（OECD，欧盟的指导方针）及毒理学研究。

MatTek公司的基于人体细胞的3D皮肤模型EpiDerm于1993年研发上市，立即获得巨大的成功。从此MatTek通过以下方式彻底改变了基于人体细胞的体外模型市场：（1）低成本（降低50-80％的成本）；（2）通过发表质量控制标准（同时具备阳性和阴性对照）以确保可为行业及监管机构接受的高可重复性水平；（3）优化研究人员的实验结果。通过创造性使用非动物的体外测试，融合降低(直接/间接)成本的强大协同效应及显著提高的产品质量，MatTek极大的降低产品成本、提高实验室效率及高质量的高可重复性产品，减少实验动物的使用，具有无可超越的长期组织可重复性；3-D仿真结构；高度分化的组织模型；代谢和分裂活性；源于正常的人体细胞；培养、操作简便；无血清培养；可定量的客观检测；物美价廉的优点。因而使得MatTek人细胞来源的体外组织模型被包括美国、加拿大、欧洲、日本和东亚的众多跨国公司与研究机构采用。

目前MatTek公司的产品主要包括EpiDerm（皮肤模型）、MelanoDerm（黑色素模型）、EpiOcular（眼周表皮模型）、EpiAirway呼吸道上皮模型）、EpiDermFT (全厚的皮肤模型) 、EpiVaginal（阴道上皮模型）、EpiOral（口腔上皮模型）、 EpiGingivalTM（牙龈上皮模型）、Psoriasis tissue（牛皮癣组织模型）及树突细胞。