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MatTek/EpiDerm™/epiderm/1 Ea
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MatTek’spatentedEpiDermsystemisaleADInginvitrotestingtechnologyfordermaltoxicologistsandformulationscientists.WithmultipleECVAMvalidationsandOECDacceptedtestguidelines,EpiDermisaproveninvitromodelsystemforchemical,pharmaceuticalandskincareproducttesting.SkinIrritation(OECDTG439)andCorrosion(OECDTG431)TestingProducedunderGMPExcellentinvitro–invivocorrelationTechnology:AlsoknowngenericallyasaReconstructedHumanEpidermis(RHE),EpiDermisaready-to-use,highlydifferentiated3Dtissuemodelconsistingofnormal,human-derivedepidermalkeratinocytes(NHEK)culturedonspeciallypreparedtissuecultureinserts.TissueCultureInsertCulturedattheair-liquidinterface(ALI),EpiDermallowsfortheevaluationoftopicallyappliedcompounds,chemicals,cosmetic/personalcareproductingredientsandfinalformulations.Withourstraightforwardprotocolsand20+yearsofdata,EpiDermisthegoldstandardforabroadrangeofhighlypredictiveinvitroapplications.EpiDermexhibitshumanepidermaltissuestructureandcellularmorphologywithgreateruniformityandreproducibility.It’s3Dstructureconsistingoforganizedandproliferativebasalcells,spinousandgranularlayers,andcornifiedepidermallayersaremitoticallyandmetabolicallyactive.Applications:TheEpiDerm3Dhumantissuemodelisusedacrossadiverserangeofapplicationsincludingsafetyandriskassessment,andBIOLOGicalefficacy.WithAir-liquidInterface(ALI)culturetechnology,EpiDermcanbeusedtoevaluatebiologicalresponsestotopicalapplicationsofformulationsoringredients.Simpleprotocolsandtheevaluationofearlycellularendpointsallowresearchtoacquiredataindays,notweeksormonths.SkinIrritation(OECDTG439)UseEpiDermtodeterminetheskinirritationpotentialofliquids,solids,gels,lotions,ointmentsorcreams.TissueviABIlityresultsareacquiredwithin3days.SkinIrritationTestProtocolSkinCorrosion(OECDTG431)Determinetheskincorrosionpotentialoftestmaterialsinasingleday.SkinCorrosionProtocolSkinHydrationMeasuretheelectricalimpedanceofEpiDermtoassesstheefficacyoftopicallyappliedmoisturizersincludingcreams,gelsandlotions.TheSkinHydrationprotocolallowsfordataanalysiswithin24hours.SkinHydrationApplicationNoteDermalDrugDeliveryUtilizeexistingtransdermalpermeationequipmentorMatTek’ssimplesingleinsertpermeationdevicestoassessAPIpermeationandflux.DeterminerelativesafetywiththeEpiDermMTTET-50assay.PercutaneousAbsorptionProtocolPhototoxicityIdentifyphototoxiceffectsoftopicalorsystemictestsubstancesusingtheEpiDermPhototoxicityAssay(pre-validated)PhototoxicityProtocolDermalGenotoxicityIdentifygenotoxiceffectsoftopicalorsystemictestsubstancesusingEpiDermGenotoxicityAssays(pre-validated)MicronucleusAssayProtocolCometAssayProtocolEpidermalDifferentiationEvaluateactivecompoundsforeffectsonepidermaldifferentiationusingtheunder-developedEpiDermtissuemodelBrowseourreferencelibrarytoseehowourEpiDermtissuehasbeenusedintheseareasofstudy. a[rel~="mtli_filesize109MB"]:after {content:" (1.09 MB)"}a[rel~="mtli_filesize79407kB"]:after {content:" (794.07 kB)"}a[rel~="mtli_filesize48179kB"]:after {content:" (481.79 kB)"}a[rel~="mtli_filesize22423kB"]:after {content:" (224.23 kB)"}a[rel~="mtli_filesize14213kB"]:after {content:" (142.13 kB)"}TechSpecs:TissueKit:AstandardEpiDermkit(EPI-200)consistsof24tissues.(Tissue“kits”containtissues,asmallamountofculturemedium,andplasticware;contactMatTekforspecifickitcontents)Formats:9mm&22mmindividualinsertsand96-wellHTSplates–tissueculturesubstrateischemicallymodifieswithaporesizeof0.4μmCulture:Air-liquidinterfaceHistology:8-12celllayersplusstratumcorneum(basal,spinous,andgranularlayers)Lotnumbers:Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualkitswithinagivenlotoftissues.Alltissuekitswithinalotareidenticalinregardstocells,medium,handling,cultureconditions,etc.Shipment:At4°Conmedium-supplemented,agarosegelsShipmentday:EveryMonday.ShipmentonThursdayalsopossIBLeuponspecialrequestDelivery:TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-ThursdaydependingonlocationShelflife:Includingtimeintransit,tissuesmaybestoredat4°Cforupto6dayspriortouse.However,extendedstorageperiodsarenotrecommendedunlessnecessary.Inaddition,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning)Lengthofexperiments:Culturescanbecontinuedforupto3weekswithgoodretentionofnormalepidermalmorphology.Culturesmustbefedeveryotherdaywith5.0mloflonglifemaintenancemedium(EPI-100-LLMM),standardmaintenancemedium(EPI-100-MM),ornewmaintenancemedium(EPI-100-NMM).Extendedcultureexperimentsrequiretheuseofculturestands(MEL-STND;seephoto)orwashers(EPI-WSHR)in6-wellplatestoallowforculturingin5.0mlofculturemediumCellsType:Normalhumanepidermalkeratinocytes(NHEK)Geneticmake-up:SingledonorDerivedfrom:Neonatal-foreskintissue(NHEK)Alternatives:NHEKfromadultbreastskinScreenedfor:HIV,Hepatitis-B,Hepatitis-C,mycoplasmaMediumBasemedium:Dulbecco’sModifiedEagle’sMedium(DMEM)Growthfactors/hormones:Epidermalgrowthfactor,insulin,hydrocortisoneandotherproprietarystimulatorsofepidermaldifferentiationSerum:NoneAntibiotics:Gentamicin5µg/ml(10%ofnormalgentamicinlevel)Anti-fungalagent:AmphotericinB0.25µg/mlpHIndicator:PhenolredOtheradditives:Lipidprecursorsusedtoenhanceepidermalbarrierformation(proprietary)Alternatives:Phenolred-free(EPI-200-PRF),antibiotic-free(EPI-200-ABF),anti-fungal-free(EPI-200-AFF),orhydrocortisone-freemediumandtissue(EPI-200-HCF)areavailable.Agentsareremovedatleast3dayspriortoshipment.PleasediscussyourspecificmediaformulationandtissueneedswithMatTekscientificpersonnelbeforeplacingyourorder.Assay/Maintenancemedium:Threemaintenancemediaareavailable.EPI-100-MMisequivalenttotheassaymediumusedforshorttermtoxicologicaltesting.LongLifeMaintenanceMedium,EPI-100-LLMM,containsα-MSHandβ-FGF.NewMaintenanceMedium,EPI-100-NMM,containsKeratinocyteGrowthFactor(KGF),butdoesnotcontainα-MSHandβ-FGF.Althoughallmediawillresultingoodretentionofepidermalmorphologyforupto3weeks(5.0mlchangedeveryotherday),useofEPI-100-NMMwillgivetheoptimalhistologyforlongtermstudies.QualityControlandSterilityVisualinspection:Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipmentEnd-usetesting:Tissuesareexposedto1%TritonX-100for4,6,8and12.5hours.Thetimeofexposurerequiredtoreducethetissueviability(ET-50)usingtheMTTviabilityassayisdetermined(SeeMatTekEpiDermMTTET-50Protocol)foreachlotoftissue.ET-50’smustfallwithintherangeofthe1996EpiDermdatabaseof4.77–8.72hours.ET-50’sincustomers’labsmaydifferslightlyfromtheMatTekresults.Sterility:Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontaminationScreeningforpathogens:AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“Biosafetyinmicrobiologicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalserviceNotificationoflotfailure:IfatissuelotfailsourQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutchargewhenappropriate.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossible(Undernormalcircumstances,ET-50failureswillbenotifiedbyWednesday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)

MatTek公司是麻省理工学院两个化学工程教授于1985成立的科技公司。1991年,该公司利用其核心的聚合物表面改性技术进入新兴的组织工程领域,目前已成长为的组织工程技术公司,致力于生产创新性的3D重构人体组织模型,该模型相较于传统2D细胞模型具有更可靠的实验结果。其生产的重构皮肤,眼部和呼吸道组织模型已广泛应用于化妆品,化工,医药和家用产品等行业的毒理学监控(OECD,欧盟的指导方针)及毒理学研究。


MatTek公司的基于人体细胞的3D皮肤模型EpiDerm1993年研发上市,立即获得巨大的成功。从此MatTek通过以下方式彻底改变了基于人体细胞的体外模型市场:(1)低成本(降低50-80%的成本);(2)通过发表质量控制标准(同时具备阳性和阴性对照)以确保可为行业及监管机构接受的高可重复性水平;(3)优化研究人员的实验结果。通过创造性使用非动物的体外测试,融合降低(直接/间接)成本的强大协同效应及显著提高的产品质量,MatTek极大的降低产品成本、提高实验室效率及高质量的高可重复性产品,减少实验动物的使用,具有无可超越的长期组织可重复性;3-D仿真结构;高度分化的组织模型;代谢和分裂活性;源于正常的人体细胞;培养、操作简便;无血清培养;可定量的客观检测;物美价廉的优点。因而使得MatTek人细胞来源的体外组织模型被包括美国、加拿大、欧洲、日本和东亚的众多跨国公司与研究机构采用。


目前MatTek公司的产品主要包括EpiDerm(皮肤模型)、MelanoDerm(黑色素模型)、EpiOcular(眼周表皮模型)、EpiAirway呼吸道上皮模型)、EpiDermFT (全厚的皮肤模型EpiVaginal(阴道上皮模型)、EpiOral(口腔上皮模型)、 EpiGingivalTM(牙龈上皮模型)、Psoriasis tissue(牛皮癣组织模型)及树突细胞。


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